Inflammatory Bowel Disease (IBD) is a chronic inflammatory disease with 2 major categories:
Ulcerative Colitis (UC) and Crohn's Disease (CD). The main features of IBD include bloody diarrhoea and associated bodyweight loss. Classification is associated with the area of GI tract affected - UC involving the colon and rectum and CD affecting any part of the GI tract (mainly ileum and colon).
For simple, cost-effective assessment of efficacy in UC models, Axis Bio offers both acute and chronic DSS induced models in mice.
- Induction starts on day 1 with 3% DSS dosed in drinking water for 5 or 7 consecutive days. A recovery period of 7 days can then be adopted. For chronic disease a 7-day 2% DSS solution on/7-day DSS solution off approach is used for 3 cycles. Please note: experimental details can be altered for a bespoke design.
- Disease progression occurs with animal bodyweight loss observed from day 2. Mice are monitored daily for the following:
- Body weight loss
- Stool consistency
- Blood in stool
- Dependent on study design/question to be answered the following end-points can be measured:
- Colon weight:BW
- Colon length
- Myeloperoxidase (MPO) activity analysis
- FACS analysis
- QPCR analysis